MAPS science team meets to discuss year one accomplishments and plans for year two

Year 1 Science Meeting Group at Konza Prairie Biological Station

     On November 16, 2018, faculty and students working on the Kansas NSF EPSCoR RII Track-1 Award OIA-1656006 titled: Microbiomes of Aquatic, Plant, and Soil Systems across Kansas (MAPS) gathered at the Konza Prairie Biological Station near Manhattan, KS to discuss accomplishments and challenges that occurred during year one as well as review and modify research plans for year two. The morning began with a welcome from Walter Dodds, a co-principal investigator (co-PI) for the MAPS project and member of aquatic systems research team. Then, the junior faculty team leaders for the aquatic, plant and soil systems research groups each gave a ten minute research update that was followed by a five minute discussion addressing challenges and the proposed research plans for year two. Discussions on project modeling and data management issues followed the team presentations. Over the lunch hour, MAPS students participated in a poster session. Twelve undergraduate and graduate students from the University of Kansas, Kansas State University and Fort Hays State University presented their research on the following MAPS topics:

• Methane Oxidation in Native Prairie Soil 
• Do novel inputs to the Kansas River affect the water or sediment microbiome and water chemistry? 
• The Effects of Climate and Land Use on Methanotropic Communities
• Plant-soil Microbiome Feedback Impacts on Native and Non-native Grasses Throughout Kansas
• Soil health across a precipitation gradient with different land uses
• Impact of Drying and Rewetting Cycles on Microbial Communities in Tallgrass Prairie
• Effects of Land Management on the Microbial Community, Soil Structure, and Nutrient Dynamics of Cultivated Grain Sorghum
• Recurrent fires do not affect the abundance of soil fungi in a frequently-burned pine savanna
• A Mechanistic Model of Plant-Symbiont Interactions
• How roots and microbes transform decaying organic matter into bioavailable phosphorus: pH as a master variable
• Time Series Transcriptomic Responses to Drought in Maize Seedings
• Impact of Land Use on Groundwater Chemistry and Microbial Communities in Great Bend Prairie Aquifers

     Breakout sessions were held in the afternoon to discuss research methods and sites locations specific to each research team, followed by a whole group discussion synthesizing year one's findings. Publications and data sharing policies made up the final session for the day. Research team members who were unable to physically attend the meeting, were able to participate in the sessions through video conferencing.

Funding for the Science Meeting was provided by the Kansas NSF EPSCoR RII Track-1 Award OIA-1656006 titled: Microbiomes of Aquatic, Plant, and Soil Systems across Kansas. The award's research, workforce development, and educational objectives are designed to enhance research capacity and STEM education in Kansas, expand the STEM workforce and prepare a new generation for STEM careers in the areas of aquatic, plant and soil microbiome environments and ecological systems.

Girl Scouts of the Kansas Heartland learn about fungi

     On Saturday, November 3, 2018, Theo Michaels and Jacob Hopkins presented a lesson module on fungi to the Girls Scouts of the Kansas Heartland during the 2018 Girl Scout STEM Expo at Camp Tongawood in Tonganoxie, KS.  Both Michaels and Hopkins are Ecology and Evolutionary Biology (EEB) PhD candidates at the University of Kansas (KU) working in the Sikes Microbial Laboratory with Dr. Benjamin Sikes, Assistant Professor of EEB at KU, Assistant Scientist at the Kansas Biological Survey (KBS), and Kansas NSF EPSCoR RII Track-1 Award OIA-1656006 titled: Microbiomes of Aquatic, Plant, and Soil Systems across Kansas (MAPS) plant systems research team member. Michaels is also a member of the MAPS research team.
     About two years ago, Andrew Mongue and Kaila Colyott, two KU EEB Graduate Student Organization (GSO) members, and some local girl scout troops collaborated to create this event. Hopkins added, “Due to their fantastic efforts and the success of the first event, the event was held again this year." Both Michaels and Hopkins were invited to participate by Anna Klompen, the current KU EEB GSO outreach committee chair, who was instrumental in planning this year's event.

Jacob Hopkins and Theo Michaels
teaching Girl Scouts about fungi

    The 2018 STEM Expo presenters were asked to develop “a module that educated scouts in particular STEM fields.” Hopkins explained, “The modules were designed to be an informative and interactive way to get scouts interested in science.” In addition, some of the modules were designed to meet badge requirements. Hopkins described the module he and Michaels taught as follows: “In our module, we taught the girls about the basics of fungi, what mushrooms are, mushroom anatomy, basic microscopy, how to recognize Kansas mushrooms, mushroom safety, and how to grow their own fungi. The fungi basics and safety sections were composed of light lecture. The mushroom anatomy was a hands-on activity where scouts identified parts of a provided mushroom (purchased at the grocery store). The Recognizing mushrooms portion relied on KU herbarium specimens to teach the scouts about edible mushrooms in Kansas and their poisonous look-a-likes. The Grow your own fungi activity allowed the girls to plate fungi from different sources (i.e. fingers, leaves, sticks, rocks, shoes) on a petri dish that they got to take home and record what grew.”
     When Hopkins and Michaels were asked why they wanted to participate in the Girl Scout STEM Expo, Hopkins said “We participated because scientific outreach opportunities are key for: a) educating the general public about what scientists do, b) showing off how wonderful the world around us is, and c) providing a strong platform for getting scouts interested in STEM fields at a young age. Also, developing and presenting outreach modules is a lot of fun.” Michaels went on to add that the activity provided “a good chance for the girl scouts to talk to real live scientists about science, what it takes to be a scientist, and how to foster their interests moving forward. It also gives them a chance to see how science is a lens by which to explore our world and ask questions that can both directly and indirectly pertain to our daily lives.”
     In addition to Hopkins’ and Michaels’ module, “there were several other STEM modules presented by KU EEB GSO students at the event.”  Multiple troops from across Kansas, made up of about 80 Girl Scouts, attended the 2018 STEM Expo.

HERS student studies the environmental impacts of bison and fire on the prairie

   

Willow Kipp

     Willow Kipp understands that becoming well informed about Native American and environmental policies is essential to be a successful advocate for her community, “instead of being reactive to the rapidly changing governments and climate change.” With this goal in mind, Willow wanted to conduct further research on environmental issues, so she decided to attend the 2018 Haskell Environmental Research Studies (HERS) Summer Program supported by the Kansas NSF EPSCoR RII Track-1 Award OIA-1656006 titled: Microbiomes of Aquatic, Plant, and Soil Systems across Kansas. 
     The HERS program, housed at Haskell Indian Nations University in Lawrence KS, “provides the platform for various stages of support for programs of interest to American Indian/Alaska native communities, most recently focused on the effects of climate change on indigenous communities.” The program allows students to conduct independent research and then present their findings at a national convention. Willow selected a project that allowed her to study the important role bison and fire play in sustaining the biodiversity of the Blackfeet Reservation's prairie. She titled her project Bison, Fire, Nitsitapii: Utilizing Innii (Bison) and Controlled burns to Maintain Short-Mixed Grass Prairie Biodiversity within the Blackfeet Nation.
     She described her research as follows: "Blackfeet people have actively managed the short-mixed grass prairie through bison herd management and controlled burns for hundreds of years.... Bison are a keystone species on the Blackfeet Reservation’s short-mixed grass prairie which is located in the Rocky Mountain Plains Region." Bison and controlled burns restore the prairie's ecological growth succession cycle (EGSC) and enhance its biodiversity. The bison's grazing habits and the bison's fur, chips, urine and wallows promote growth and productivity of the prairie's indigenous plants. Thus,"highlighting bison as caretakers of the land." Exercising a similar function,"controlled burns regenerate the ecological growth succession cycle too and reduce the intensity and destructiveness of wild fires" by burning the vegetation that can fuel them. Bison also "graze on burned sites which is one way bison and fire" work together to maintain the biodiversity within the Blackfeet Reservation's short-mixed grass prairie. Willow concluded, "I found that when both bison and fire are being actively managed, the prairie is in its healthiest state and biodiversity is sustained.” This past summer, Willow presented her research during a poster session at the University Corporation of Atmospheric Research (UCAR) 2018 Conference held in Boulder, CO.

Willow's poster describing her HERS research
 that she presented at UCAR

    When she described her HERS experience, Willow said the program provided her with a great opportunity to conduct "in depth research on a topic of interest to me and my community.” She added that it also allowed her to enhance her writing skills. She mentioned the best and most eye opening experience came when the 2018 HERS cohort visited the Konza Prairie Biological Station. During the visit, Willow had the opportunity to personally observe bison, conduct field research, and use the data collected for her final project.  In addition, she said the HERS program allowed her to “create a scholarly project documenting ancient tribal practices as a current and relevant necessity of today.”
    Willow is Shoshone-Bannock from Fort Hall, ID and Blackfeet from Browning MT.  She earned her bachelor's and associate's degree in Native American Studies (NAS) and Environmental Studies (ENST) from the University of Montana in Missoula MT. In addition to her studies, Willow chaired several campus wide events. She also was a member of the Sacred Roots Language Society, and she won the University of Montana's Student Diversity and Leadership Award. As for her future plans, Willow wants to attend law school and pursue a career in Tribal/criminal law working for the Blackfeet or Shoshone/Bannock tribes.

Workforce Development, Education and Outreach funding for the HERS program is provided by the Kansas NSF EPSCoR RII Track-1 Award OIA-1656006 titled: Microbiomes of Aquatic, Plant, and Soil Systems across Kansas. The award's workforce development and educational objectives are designed to enhance STEM education in Kansas by supporting activities that will lead to an expanded STEM workforce or prepare a new generation for STEM careers in the areas of aquatic, plant and soil microbiome environments and ecological systems.

Benedictine College student studies the impacts of fire severity on fungi

Hannah Dea

     When Hannah Dea took a Mycology course at Benedictine College, taught by Dr. Janet Paper, she became “fascinated by the enormous role that fungi play in the life and health of plants.” She especially enjoyed learning about mycorrhizal fungi, the fungi that form a mutualist relationship with the root systems of plants as well as provide plants with nutrients. Because of this course, she wanted to continue researching ecological topics, so she found and decided to apply to the 2018 Ecology and Evolutionary Biology (EEB) summer research experience for undergraduates (REU) at the University of Kansas (KU).

     Upon her acceptance into the EEB REU program, Hannah chose a fungi research project that paired her with Dr. Benjamin Sikes, Assistant Professor of EEB at KU, Assistant Scientist at the Kansas Biological Survey, and Kansas NSF EPSCoR RII Track-1 Award OIA-1656006 titled: Microbiomes of Aquatic, Plant, and Soil Systems across Kansas plant systems research team member. She titled her project Fire severity effects on ectomycorrhizal colonization of Longleaf pine and Loblolly pine. Hannah explained her research project as follows:

Hannah analyzing ectos roots

“My project looked at how fire severity affects the relationship between microbial communities and soil nutrients and how this in turn affects a fire tolerant and a non-fire tolerant species of pine. I wanted to find out whether a more severe fire, which would kill off the microbial communities and release nutrients from the soil, would make the soil conditions more favorable to the fire tolerant Longleaf pine or to the non-fire tolerant Loblolly pine. My hypothesis was that, since fire tolerant species are more accustomed to fire affected soils, the fire tolerant species would be benefited by increasing fire severity while non-fire tolerant species would not. To test this, after both species were grown in high, medium, and low severity fire soil for 3 months, I took the percent colonization of ectomycorrhizae (a fungal mutualist on the plant roots essential for nutrient uptake) on both species of pine as well as the biomass of each plant. I found that while the biomass of the two species did not differ, the Longleaf pine had a much more efficient relationship with the ectomycorrhizae than the Loblolly pine. This showed that the fire tolerant species had a bit of an advantage over the non-fire tolerant species."

     When she was asked what the best part of her summer research experience was, she replied “My favorite part of the experience was living in a research community that allowed me to focus on sharpening my research skills. There were so many resources at KU that allowed me to do science without hindrance from lack of help or resources. I love plant and fungal ecology, and this allowed me to dive into it for a whole summer!” She added that the experience taught her how to conduct research, create good questions, make predictions, collect and analyze data and to communicate science “in a way that even non-scientists would understand.”

     Hannah is from Templeton, Iowa and is majoring in Biology with a minor in Latin at Benedictine College in Atchison, KS. She is a student leader involved with Campus Ministries, and she assists with organizing and setting up masses on campus. Hannah has also been an officer in the Biology Club and is currently a Latin tutor for the college. Participating in this EEB REU reaffirmed her desire to pursue a career as a conservation biologist, an educator, and/or a naturalist. She plans to earn her masters degree in ecology after graduation. 

Kansas State University MAPS researchers receive award from Office of Biological and Environmental Research in the Department of Energy

Dr. Jesse Nippert and Dr. Lydia Zeglin
KSU

   The Office of Biological and Environmental Research in the Department of Energy has awarded nearly $1 million dollars to two Kansas NSF EPSCoR RII Track-1 Award OIA-1656006 titled: Microbiomes of Aquatic, Plant and Soil Systems across Kansas (MAPS) researchers from Kansas State University (KSU). Dr. Jesse Nippert, associate professor of biology at KSU and part of the MAPS plant systems focus team, and Dr. Lydia Zeglin, assistant professor of biology at KSU and part of the MAPS acquatic systems focus team, plan to combine observational, experimental and modeling approaches in an effort to enhance the predictability of ecosystem consequences related to shrub encroachment and drought in the Great Plains region. The title of their project is DE-SC001109037: Using root and soil traits to forecast woody encroachment dynamics in mesic grassland. They will also be working with collaborators Kate McCulloh, assistant professor at the University of Wisconsin-Madison, and Kevin Wilcox, assistant professor at the University of Wyoming. The team will conduct experiments and collect their data at the Konza Biological Field Station. They will use root and soil traits, taken at various soil depths, which contain microbes, water and a large amount of carbon to forecast the plant encroachment dynamics associated with grasslands that receive moderate amounts of precipitation. The data collected and the results from this project “will define the depth-resolved feedbacks of drought and dominant vegetation on below ground root architecture, soil microbial carbon cycling, and ecosystem carbon balance.”

For additional information regarding the proposal go to: DE-SC001109037
Click here for the KSU press release

ANNOUNCING MAPS First Award Funding Opportunity

     Kansas NSF EPSCoR is announcing a funding opportunity for First Awards in the areas related to the current Kansas NSF EPSCoR focus of microbiomes as broadly construed to be in aquatic, plant and/or soil systems. The First Award program helps early career faculty become competitive for funding from the research directorates at the National Science Foundation. 

The full request for proposals with submission instructions can be downloaded as a PDF at: http://www.nsfepscor.ku.edu/funding.html

Submission Deadlines:

     Letters of Intent due by 5:00 pm on Wednesday, October 31, 2018.

     Full proposals due by 5:00 pm on Thursday, December 20, 2018 

Please note new proposal submission details included in the RFP.

Eligible to apply is any individual tenure track faculty member who:

• is currently untenured at the assistant professor rank at Kansas State University, University of Kansas, Wichita State University, Emporia State University, Fort Hays State University, Pittsburg State University or Washburn University; 

• is within the first three years of his/her faculty appointment;

• has not received a previous First Award or similar funding from another EPSCoR or EPSCoR-like (Centers of Biomedical Research Excellence, COBRE) program in Kansas; and

• is not currently be nor previously been a lead Principal Investigator of a research grant funded by a federal agency.

In addition, one of the following conditions must apply:

• The Principal Investigator has a pending proposal or is planning to submit a proposal to the NSF (or other federal funding agency) for the proposed research submitted to this program.  If in the planning stages, the proposed research must be submitted to a federal funding agency by July 31, 2020. 

• The Principal Investigator has had the proposed research declined by the NSF (or other federal funding agency) and has a plan to re-submit the proposed research by July 31, 2020. 

Only projects with research in areas that are related to the current Kansas NSF EPSCoR focus of microbiomes as broadly construed to be in aquatic, plant and/or soil systems are eligible for First Awards.

Workforce Development, Education and Outreach funding for the MAPS first awards is provided by the Kansas NSF EPSCoR RII Track-1 Award OIA-1656006 titled: Microbiomes of Aquatic, Plant, and Soil Systems across Kansas. The award's workforce development and educational objectives are designed to enhance STEM education in Kansas by supporting activities that will lead to an expanded STEM workforce or prepare a new generation for STEM careers in the areas of aquatic, plant and soil microbiome environments and ecological systems.

NSF-FUNDED GRADUATE POSITIONS AT KANSAS STATE UNIVERSITY IN MICROBIOMES OF AQUATIC, PLANT OR SOILS (MAPS)

     The Division of Biology at Kansas State University is recruiting diverse, highly-qualified graduate students to assist with understanding the linkages among microbiomes of aquatic, plant and soil (MAPS) ecosystems across the state ofKansas. 

The goal of MAPS is to understand:

1. How microbiome structure and function among these systems change across the precipitation gradient of Kansas and land use, and 
2. How those changes in microbiomes affect broader community and ecosystem properties. In all, the integrated and collaborative NSF-funded project is driven by >15 investigators, all of whom work collaboratively and train students in an interdisciplinary framework.

     If you are interested in developing skills in collaborative, team-based science focused on deploying cutting-edge tools in environmental microbiology and informatics, please contact the listed individuals who might serve as your graduate advisor. Students must discuss their interests with a potential advisor (by email or by scheduling a phone conversation by email) prior to submitting an application. 

• For Biology see https://www.k-state.edu/biology/grad/why.html; 
• For Agronomy see http://www.agronomy.k-state.edu/graduate/admissions/index.html; 
• For Plant Pathology see https://www.plantpath.k-state.edu/graduate/application-information/index.html).  We encourage students with experience in bioinformatics, molecular ecology, microbiology, field work and experimental design to apply. Experience working across multiple ecosystems or across environmental gradients will be preferred but not required.

Applications due by 

15 December for Fall or Summer 2019 start.

MAPS NSF Award information: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1656006

Agronomy:

• Chuck Rice (Soil Microbiology; http://www.agronomy.k-state.edu/people/faculty/rice-charles/)

Biology:

• Walter Dodds (Aquatic Ecology; http://www.k-state.edu/doddslab/)
• Ari Jumpponen (Fungal Ecology; https://www.k-state.edu/biology/people/tenure/jumpponen/index.html)
• Sonny Lee (Metagenomics, bioinformatics; http://www.k-state.edu/biology/people/tenure/stmlee)
• Thomas Platt (Microbial Evolution; http://www.platt-lab.com/)
• Lydia Zeglin (Microbial Ecology; https://www.k-state.edu/biology/people/tenure/zeglin/index.html)

Plant Pathology

• Sanzhen Liu (Bioinformatics; http://www.plantpath.k-state.edu/people/faculty/liu/)

Workforce Development, Education and Outreach funding for these MAPS graduate positions is provided by the Kansas NSF EPSCoR RII Track-1 Award OIA-1656006 titled: Microbiomes of Aquatic, Plant, and Soil Systems across Kansas. The award's workforce development and educational objectives are designed to enhance STEM education in Kansas by supporting activities that will lead to an expanded STEM workforce or prepare a new generation for STEM careers in the areas of aquatic, plant and soil microbiome environments and ecological systems