KS-LSAMP student studies agrobacterial genetic diversity

 

Veronica collecting samples from the Konza Prairie

     Veronica Mateo, a recent graduate of Dodge City Community College  (DC3) in Dodge City, Kansas, considers the well being of animals her passion. She commented, "Ever since I was a child I would find myself tending to the care and needs of my family pets." Caring for animals and knowing she could impact their lives was the main reason she decided to pursue an Associate’s degree in Wildlife Biology. Once she graduated, she knew she wanted to continue her education at Kansas State University (KSU).  So, she was excited to learn that during her transition to KSU, she had an opportunity to participate in the Research Immersions Pathways to STEM (RiPs) program.
     The RiPs program is a summer research experience offered by the Pathways to STEM: Kansas Louis Stokes Alliance for Minority Participation (KS-LSAMP) at KSU that provides students with a unique opportunity to work with faculty and conduct independent research. LSAMP is a National Science Foundation (NSF) program that recruits, supports, and encourages underrepresented minority students to pursue baccalaureate degrees in Science, Technology, Engineering and Math (STEM) fields. The KS-LSAMP RiPs program is specifically for Kansas community college students who intend to transfer to KSU or who are current KSU sophomores and juniors with no prior research experience.
     This summer, Veronica's research interests expanded to include the plant microbiology associated with ecosystems. She explained that because “Plants play a huge role in an animal’s ecosystem, the type of research I am interested in deals with plant pathogens.” Dr. Thomas Platt, Assistant Professor of Biology at KSU and a member of the Kansas NSF EPSCoR RII Track-1 Award OIA-1656006 titled: Microbiomes of Aquatic, Plant, and Soil Systems across Kansas plant systems research team, was her mentor. Veronica titled her project, the Degree of Agrobacterial Genetic Diversity within an infected plant and describes her research project as follows: “Agrobacterium tumefaciens are generally found in the root environment of the plants known as rhizosphere. Because interactions among different strains can influence pathogen dynamics, we aimed to determine the degree of agrobacterial diversity co-occurring on a single host. A typical diseased plant will harbor a gall or tumor on the crown. The samples were collected from Konza Prairie. We plated the samples collected onto a semi-selective media to identify and isolate biovar 1 agrobacteria seen as black, shiny colonies. We then used a biochemical test, then streak purified and preserved 360 isolates from these plants so that we could characterize the phenotypic and genetic attributes of the agrobacteria present. We used PCR to amplify and subsequently sequence the recA locus of the isolates from the infected sunflower to determine if the infected plant was colonized by one or several agrobacterial genotypes. We used a phylogenetic analysis that includes representatives of all 11 of the known genomovars of A. tumefaciens to determine which group or groups of agrobacteria were present. In the future we would like to determine if co-occurring genotypes significantly impact each other’s fitness. However, we are currently troubleshooting the recA locus at the moment.”

Veronica, Dr. Platt
and colleagues

     When asked what she learned from her KS-LSAMP RiPs experience, Veronica said, “Previously, I had never conducted actual research. The lab experience is definitely different from my science experience at the community college or even high school. You don’t always get the results right away, and you will be let down sometimes. However, that is science. We have to learn from our mistakes and analyze what went wrong. This research taught me, overall, about what a research lab experience is and how to be patient.” Her favorite part of the summer experience was working in the lab and meeting the people associated with Dr. Platt’s lab. She added, “When I had any issues or questions I did not hesitate to ask them and having that resource made it an enjoyable experience.”
     Veronica is from Dodge City, Kansas, and is currently a junior majoring in Biology at KSU. While studying at KSU, she plans to continue her research in Dr. Platt’s lab. As for her future plans, she said, “My career plan is to apply to the Veterinary program here at Kansas State University over the summer of 2019 and someday work in an environment surrounded by animals.”

Workforce Development, Education and Outreach funding for the KS-LSAMP  program is provided by the Kansas NSF EPSCoR RII Track-1 Award OIA-1656006 titled: Microbiomes of Aquatic, Plant, and Soil Systems across Kansas. The award's workforce development and educational objectives are designed to enhance STEM education in Kansas by supporting activities that will lead to an expanded STEM workforce or prepare a new generation for STEM careers in the areas of aquatic, plant and soil microbiome environments and ecological systems

MAPS Researcher receives NSF Early Career Investigator Award in Plant Genome Research

Dr. Sanzhen Liu
KSU

    Sanzhen Liu, a research team member on theMicrobiomes of Aquatic, Plant and Soil Systems across Kansas (MAPS) project and an assistant professor in the plant pathology department at Kansas State University (KSU), has received a four-year 2.4 million dollar NSF Early Career Investigator Award in Plant Genome Research. The title of the research project is Under the Hood: The Genetic Components of Maize Transformation, (NSF IOS ECA-PGR Award #1741090). Liu will be collaborating with Sunghun Park, professor of horticulture and natural resources at KSU, Frank White from the University of Florida, Myeong-Je Cho from the University of California-Berkeley, and Hairong Wei from Michigan Technological University. The study seeks to "understand the genetic basis underlying the ability of plant tissues to regenerate into whole plants."
      Specifically, Liu and his team will investigate the genome engineering of maize. They selected maize because it is one of the highest-yielding cereal crops in the world that faces challenges of dramatic yield increases, "particularly under highly variable climates and disease pressures." The researchers plan to sequence the genome of an amenable maize tissue culture to identify the genetic elements that regulate culture ability. The group will develop and apply novel approaches in order to decode the complex maize genome.  Specifically, the team will utilize a plant-bacterium delivery system that enables "plants to gain benefits from the bacterium."  Then, a "designable bacterial system specifically interacting with genes of interest in the maize genome will be utilized to study gene function and manipulate cell development."
    This investigation will also provide training in genetics and computation as well as large data education for students and post docs involved in the project. In addition, it "will collaborate with the Kansas Louis Stokes Alliance for Minority Participation to encourage involvement of historically underrepresented students in STEM fields."

HERS student examines wetland viability

   

Tasha Chenot presenting her research at UCAR, 
Boulder, CO.

     As a recent Environmental Science graduate from Haskell Indian Nations University, Natasha (Tasha) Chenot saw the Microbiomes of Aquatic, Plant, and Soil Systems across Kansas (MAPS)  partnership with the Haskell Environmental Research Summer Internship (HERS) program as an opportunity to pursue her research interests associated with climate change and the resiliency of Indigenous communities. Specifically, she was interested in investigating the "interplays between culture, identity, and the environment" as it related to Indigenous geographies, environmental policy, and environmental stewardship. As an undergraduate, these interests led her to join the Haskell Indian Nations University Tribal Eco-Ambassadors Program sponsored by the United States Environmental Protection Agency (EPA).  While serving as a Tribal Eco-Ambassador, Tasha organized several Haskell Wetland Clean-Up events and recognized that there were only a limited number of studies related to the impacts of road construction on wetlands.  Consequently, when it came time to select a research topic for the HERS program, she decided to explore the impact of highway construction and use on the Wakarusa Wetlands, located in Lawrence, Kansas.  She titled her project, The Effects of Highway Construction and Use on the Water Chemistry of Adjacent Wetlands and explained the rationale and results of her research as follows: “Wetlands are highly sensitive to disturbances associated with highway construction and use. However, few studies have examined the during-and-after effects of highway construction on wetland viability. At the Wakarusa Wetlands, located in Lawrence, Kansas, a four-lane highway was built from May 2014 to June 2016 across the northern part of the landscape. In the summer of 2018, I worked with data collected during two time periods to assess the during-and-after effects of highway construction on the water chemistry of the Wakarusa Wetlands. Although the data was discontinuous, immediate results from water quality tests suggested that disturbance from highway construction to date has increased turbidity and decreased DO content and conductivity in the wetlands. Future research activities may include establishing a long-term, continuous monitoring system in order to further investigate changes in wetland water quality.” Tasha presented her research findings at the University Corporation of Atmospheric Research (UCAR) 2018 Conference held in Boulder, CO this past July.
     Tasha is a citizen of the Pawnee Nation of Oklahoma and is originally from Oklahoma City, OK; however, she grew up in Lawrence, KS. Her favorite part of the HERS internship was “learning about other interns’ research projects. Everyone’s project spoke to issues that they felt passionate about and centered on the cultural survival of their (and others’) community. I truly learned so much.” In addition, she said that through this internship experience, “I learned several writing and data management skills that will undoubtedly help me in my future studies.”
     Currently, Tasha is a graduate student at the University of Kansas working on a master’s degree in Geography.  Her long term goal is to move to Alaska after graduation and work as a Geographic Information Systems (GIS) Environmental Consultant for the many Alaska Native corporations located throughout the state.

Workforce Development, Education and Outreach funding for the HERS program is provided by the Kansas NSF EPSCoR RII Track-1 Award OIA-1656006 titled: Microbiomes of Aquatic, Plant, and Soil Systems across Kansas. The award's workforce development and educational objectives are designed to enhance STEM education in Kansas by supporting activities that will lead to an expanded STEM workforce or prepare a new generation for STEM careers in the areas of aquatic, plant and soil microbiome environments and ecological systems.

NSF 2026 IDEA MACHINE Competition

Kansas NSF EPSCoR is excited about this new opportunity for the community to take an active role in shaping the future direction of NSF. The NSF 2026 Idea Machine contest is a competition to identify new directions for future research. The key points of the competition are for entrants to suggest "grand challenge" questions for future research, first in narrative form and then through video "pitches." 

Authors of the best ideas will receive public recognition and/or cash prizes. Contestants much be at least 14 years old at the time of entry.

Entries will be accepted between August 31 through October 26, 2018.  Register online 

Please help us to spread the word! 

NSF EPSCoR RII Track-2 FEC Solicitation

   The NSF Established Program to Stimulate Competitive Research (NSF EPSCoR) has released the Research Infrastructure Improvement Track-2: Focused EPSCoR Collaborations (RII Track-2 FEC), NSF 18-589 solicitation.  Proposals submitted for this FY19 RII Track-2 FEC competition must address the NSF Harnessing the Data Revolution scientific topic area.  Harnessing the Data Revolution is one of the NSF Ten Big Ideas. The research proposal must address a specific compelling problem related to a scientific topic of national importance.  Details about the requirements are provided in the solicitation.  Only one proposal from each submitting organization can be submitted.

Letters of Intent are due: November 26, 2018

Full Proposals are due: January 25, 2019

Questions regarding the new RII Track-2 FEC solicitation may be directed to Dr. J.D. Swanson (jswanson@nsf.gov; 703-292-2898).

Collaboration between Kansas State University, Oklahoma State University and Langston University seeks to boost crop yields to feed a growing world population

Dr. Stephen Welch, KSU; Dr. Phillip Alderman, OSU; Dr. Franklin Fondjo Fotou, LU

Stephen Welch, professor of agronomy at Kansas State University (KSU), Phillip Alderman, assistant professor of agronomy at Oklahoma State University (OSU), and Franklin Fondjo Fotou, assistant professor and chair of the department of technology at Langston University (LU) in Langston OK, have received a RII Track-2 FEC NSF EPSCoR four-year, $4 million award.  The project, titled Building Field-Based Ecophysiological Genome-to-Phenome Prediction will study methods to improve crop yields, crop breeding programs, and in-field management using wheat as the example crop. The team plans to develop computer models and supporting data systems that combine crop physiology and genetics with actual environmental measurements such as canopy temperatures, soil profiles, and development phases.  They will use the new computer models and data systems to predict how wheat will perform in different environments. In addition, these models and data systems will be used to predict crop traits as well as provide insight for on-farm crop management and food security.  

For more information about the award go to:  RII Track -2 FEC EPSCoR OIA - 1826820

University of Puget Sound student studies impact of soil microbial communities on native Kansas grasses

Ben Papadopoulos (left); and Ben holding a snake 
during an REU fieldtrip to the KU Field Station

     Ben Papadopoulos is a biology major and math minor at the University of Puget Sound in Tacoma, WA. He first became interested in soil-plant relationships and organisms, like arbuscular mycorrhizal fungi, when they were mentioned in some of his core biology classes. Because he enjoys opportunities to engage in science projects, when he heard about the chance to “dig into” soil-plant microbe relationships as part of the department Ecology & Evolutionary Biology (EEB): Research Experience for Undergraduates (REU) program at the University of Kansas (KU), he had to apply. Ben worked with Dr. James Bever, Distinguished Foundation Professor in Ecology and Evolutionary Biology, Senior Scientist with the Kansas Biological Survey, and Lead Investigator on the Kansas NSF EPSCoR OIA-1656006 RII Track-1: Microbiomes of Aquatic, Plant, and Soil Systems across Kansas (MAPS) Plant Team, to develop his research project titled: The influence of historic precipitation regimes & land-use history on soil microbial mediated plant drought tolerance. The focus of Ben's research was to determine “whether soil microbial communities from historically dry climates enhance drought response in plants more than microbes from wet climates, and how differing land uses alters those relationships.” 

Kansas grass specimens inoculated with 
various whole soil microbial communities 

     Ben further explained his research project this way: “Climate change threatens stability of important human land use needs by decreasing the frequency of precipitation events. Given the implication that drought might have for plant productivity and diversity, it is important to understand the impact of climate change and land use on future natural and agroecosystems. Soil microbial communities play a pivotal role in mediating key ecosystem processes and driving plant productivity and diversity. Agricultural practices alter microbial communities, reducing the ability of agricultural soils to mitigate disturbances which are exacerbated under altered precipitation regimes. Understanding how undisturbed microbial communities mediate drought stress may be key to securing future food production. We used the precipitation gradient of Kansas, USA and systems varying in historical land use as a proxy for understanding how microbial communities confer drought stress in plants. We examined 1) how microbes across different precipitation and land use histories influence plant growth response; 2) the ways in which microbes from low precipitation gradients mitigate drought stress in plants; and 3) how land use history may alter this interaction. To examine these questions, we tested three species of grasses from native, resorted and agricultural systems inoculated with whole soil microbial communities from different historic precipitation and land use conditions in a full factorial design. Plants were measured for growth over time and compared across groups. We also collected fungal hyphal density for all inoculation types, indicating a general trend of increasing density with decreasing disturbance. This information will help shape productive land management strategies in a changed world.” The findings of his research revealed “that the agricultural plant, sorghum, showed different reactions to the various soils after only 3 weeks of growth where the other species did not, and that little bluestem does much better in live soils versus our sterile control.” Through this summer's research experience, Ben said he learned more about how to plan, start, and write a research project, especially as adjustments needed to be made. Plus, he added, “I also learned more about plant diversity, grasses, mechanisms of drought response and microbes!”
     Ben returns to the University of Puget Sound as a senior this fall.  Originally, Ben is from Denver, CO, and he says Tacoma WA is one of his favorite places to live. In addition to participating in this summer's research program, Ben has completed an Ecology study abroad program in Costa Rica, is a member of the Phi Sigma biological honor society, and is a Teaching Assistant. As for Ben's co-curricular involvement, he is a member of the varsity crew team, a member of the Sigma Alpha Epsilon Washington Gamma chapter, and serves on a university faculty-student committee. Currently, Ben is researching PhD programs as well as specialized masters programs with the hope of pursuing a future career in research.

Workforce Development, Education and Outreach funding for the Summer MAPS REU program is provided by the Kansas NSF EPSCoR RII Track-1 Award OIA-1656006 titled: Microbiomes of Aquatic, Plant, and Soil Systems across Kansas.  The grant's workforce development and educational objectives are designed to enhance STEM education in Kansas by supporting activities that will lead to an expanded STEM workforce or prepare a new generation for STEM careers in the areas of aquatic, plant and soil microbiome environments and ecological systems.